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Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome.  As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays

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Steve Russell, Lisa A. Meadows, Roslin R. Russell

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Systems biology is a critical emerging field that quantifies and annotates the complexity of biological systems in order to construct algorithmic models to predict outcomes from component input. Applications in medicine are revolutionizing our understanding of biological processes and systems.

Systems Biomedicine is organized around foundations, computational modeling, network biology, and integrative biology, with the extension of examples from human biology and pharmacology, to focus on the applications of systems approaches to medical problems. An integrative approach to the underlying genomic, proteomic, and computational biology principles provides researchers with guidance in the use of qualitative systems and hypothesis generators. To reflect the highly interdisciplinary nature of the field, careful detail has been extended to ensure explanations of complex mathematical and biological principles are clear with minimum technical jargon.
Organized to reflect the important distinguishing characteristics of systems strategies in experimental biology and medicine Provides precise and comprehensive measurement tools for constructing a model of the system and tools for defining complexity as an experimental dependent variable Includes a thorough discussion of the applications of quantitative principles to biomedical problems

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Agricultural Systems: Agroecology and Rural Innovation for Development

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Agricultural Systems: Agroecology and Rural Innovation for Development is a comprehensive source for developing sustainable farming systems. With the inclusion of research theory and examples using the principles of cropping system design, students will gain a unique understanding of the technical, biological, ecological, economic, and sociological aspects of farming systems science for rural livelihoods.

Editors Snapp and Pound provide a much-needed synthetic overview of the emerging area of agroecology applications to transforming farming systems and supporting rural innovation. A companion website for training and teaching features learning modules, student exercises, case studies, illustrative power point presentations, and reference links.

The wide range of subjects, integrated references, and companion website, make this core reading for courses in international agricultural systems and management, sustainable agricultural management, and cropping systems.
Coverage provides students with an enhanced understanding of how research can be harnessed for sustainable agriculture Incorporates social, biological, chemical, and geographical aspects important to agroecology Addresses social and development issues related to farming systems Companion Website for training and teaching: learning modules, student exercises, case studies, illustrative power point presentations, and reference links

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This fourth edition of this successful textbook succinctly presents the engineering concepts and unit operations used in food processing, in a unique blend of principles with applications.  Depth of coverage is very high.  The authors use their many years of teaching to present food engineering concepts in a logical progression that covers the standard course curriculum.  Both are specialists in engineering and world-renowned.  Chapters describe the application of a particular principle followed by the quantitative relationships that define the related processes, solved examples and problems to test understanding. Supplemental processes including filtration, sedimentation, centrifugation, and mixing Extrusion processes for foods Packaging concepts and shelf life of foods Expanded information on Emerging technologies, such as high pressure and pulsed electric field; Transport of granular foods and powders; Process controls and measurements; Design of plate heat exchangers; Impact of fouling in heat transfer processes; Use of dimensional analysis in understanding physical phenomena

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R. Paul Singh, Dennis R. Heldman, R. Paul Singh

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The New Public Health has established itself as a solid textbook throughout the world. Translated into 7 languages, The New Public Health distinguishes itself from other public health textbooks, which are either highly locally oriented or, if international, lack the specificity of local issues relevant to students understanding of applied public health in their own setting. Following the gold standard of knowledge set by the Council for Education in Public Health, the new edition includes:
40% new material, including all new tables, figures, data, and chapter bibliographies Updates based on the 2005 accreditation criteria of the Council for Education in Public Health (CEPH), as will feedback received from an extensive survey of professors using NPH1 Multiple case studies, chapter-ending bibliographies, and recommended readings
The second edition of The New Public Health provides a unified approach to public health appropriate for all masters level students and practitioners specifically for courses in MPH programs, community health and preventive medicine programs, community health education programs, community health nursing programs, as well as programs for other medical professionals such as pharmacy, physiotherapy, and other public health courses. Specific courses include: Fundamentals of Public Health, Introduction to Public Health Policy, Philosophy of Public Health, History of Public Health, Public Health and Healthcare Management, New Technologies and Public Health, Genetics and Biotechnologies, Bio-preparedness and others.

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Theodore H. Tulchinsky, Elena A. Varavikova

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The preservation processes for foods have evolved over several centuries, but recent attention to non-thermal technologies suggests that a new dimension of change has been initiated.The new dimension to be emphasized is the emerging technologies for preservation of foods and the need for sound base of information to be developed as inputs for systematic process design. The focus of the work is on process design, and emphasizes the need for quantitative information as inputs to process design.The concepts presented build on the successful history of thermal processing of foods and use many examples from these types of preservation processes. Preservation of foods by refrigeration, freezing, concentration and dehydration are not addressed directly, but many of the concepts to be presented would apply. Significant attention is given to the fate of food quality attributes during the preservation process and the concept of optimizing process parameters to maximize the retention of food quality. Focuses on Kinetic Models for Food Components Reviews Transport Models in Food Systems Asseses Process Design Models

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PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.

Key Features
* Focuses on gene discovery, genomics, and DNA array technology
* Covers quantitative PCR techniques, including the use of standards and kinetic analysis
includes statistical refinement of primer design parameters
* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR
Entries provide information on:
* Nomenclature
* Expression
* Sequence analysis
* Structure and function
* Electrophysiology
* Parmacology
* Information retrieval

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Michael A. Innis, David H. Gelfand, John J. Sninsky

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The Chlamydomonas Sourcebook: Cell Motility and Behavior

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This volume reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport, and more will use this reference as a guide for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a Website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility.

This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually.
Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process Essential reference to a model species for the study of mechanisms of motility in free living cells Includes methods for Chlamydomonas motility research Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action

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This comprehensive, full color hematopathology reference book emphasizes immunophenotpic features, cytogenetic studies, and diagnostic molecular aspects. Hematopathology begins with introductions to morphologic evaluation of the hematopoietic tissues and principles of immunophenotyping, cytogenetics and molecular studies followed by chapters dedicated to different types of hematologic disorders. Each chapter starts with a basic overview of hematopathlogy followed by a comprehensive review of immunophenotypic, cytogenetic and molecular findings. The text is balanced with large numbers of full color images, graphs, charts, and tables to assist the reader in understanding these highly technical issues. Emphasizes the immunophenotypic features, cytogenetic studies, and diagnostic molecular aspects of hematology Features hundreds of images, charts and tables for the identification of hematologic disorders not only based on histopathologic features, but also with the use of advanced accessory techniques

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Faramarz Naeim, P. Nagesh Rao, Wayne W. Grody

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The Chlamydomonas Sourcebook: Organellar and Metabolic Processes

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This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism Includes contributions from leaders in particular areas of research Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media Assists researchers with common laboratory problems such as contamination Includes valuable student demonstrations and properties of particular strains and mutants Edited by the leading researcher in Chlamydomonas science

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Written by an immunologist, A History of Immunology traces the concept of immunity from ancient times up to the present day, examining how changing concepts and technologies have affected the course of the science. It shows how the personalities of scientists and even political and social factors influenced both theory and practice in the field. With fascinating stories of scientific disputes and shifting scientific trends, each chapter examines an important facet of this discipline that has been so central to the development of modern biomedicine. With its biographical dictionary of important scientists and its lists of significant discoveries and books, this volume will provide the most complete historical reference in the field. Written in an elegant style by long-time practicing immunologist Discusses the changing theories and technologies that guided the field Tells of the exciting disputes among prominent scientists Lists all the important discoveries and books in the field Explains in detail the many Nobel prize-winning contributions of immunologists

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The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use

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Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants.

This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually.
Expanded revision of gold standard reference Includes latest advances in research, including completion of the genome Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields Available as part of a 3-Volume Set or sold individually

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Elizabeth H. Harris, Elizabeth H Harrisduke

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For microbiology and environmental microbiology courses, this leading textbook builds on the academic success of the previous edition by including a comprehensive and up-to-date discussion of environmental microbiology as a discipline that has grown in scope and interest in recent years. From environmental science and microbial ecology to topics in molecular genetics, this edition relates environmental microbiology to the work of a variety of life science, ecology, and environmental science investigators. The authors and editors have taken the care to highlight links between environmental microbiology and topics important to our changing world such as bioterrorism and national security with sections on practical issues such as bioremediation, waterborne pathogens, microbial risk assessment, and environmental biotechnology.
WHY ADOPT THIS EDITION? New chapters on:
Urban Environmental Microbiology Bacterial Communities in Natural Ecosystems Global Change and Microbial Infectious Disease Microorganisms and Bioterrorism Extreme Environments (emphasizing the ecology of these environments) Aquatic Environments (now devoted to its own chapter- was combined with Extreme Environments)
Updates to Methodologies:
Nucleic Acid -Based Methods: microarrays, phyloarrays, real-time PCR, metagomics, and comparative genomics Physiological Methods: stable isotope fingerprinting and functional genomics and proteomics-based approaches Microscopic Techniques: FISH (fluorescent in situ hybridization) and atomic force microscopy Cultural Methods: new approaches to enhanced cultivation of environmental bacteria Environmental Sample Collection and Processing: added section on air sampling

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Ian Pepper, Charles P. Gerba, Terry Gentry, Raina M. Maier

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Structural Biology Using Electrons and X-rays

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Structural Biology Using Electrons and X-Rays discusses the diffraction and image-based methods used for the determination of complex biological macromolecules. The book focuses on the Fourier transform theory, which is a mathematical function that is computed to transform signals between time and frequency domain. Composed of five parts, the book examines the development of nuclear magnetic resonance (NMR), which allows the calculation of the images of a certain protein. Parts 1 to 4 provide the basic information and the applications of Fourier transforms, as well as the different methods used for image processing using X-ray crystallography and the analysis of electron micrographs. Part 5 focuses entirely on the mathematical aspect of Fourier transforms. In addition, the book examines detailed structural analyses of a specimens symmetry (i.e., crystals, helices, polyhedral viruses and asymmetrical particles).

This book is intended for the biologist or biochemist who is interested in different methods and techniques for calculating the images of proteins using nuclear magnetic resonance (NMR). It is also suitable for readers without a background in physical chemistry or mathematics.
Emphasis on common principles underlying all diffraction-based methods Thorough grounding in theory requires understanding of only simple algebra Visual representations and explanations of challenging content Mathematical detail offered in short-course form to parallel the text

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Toxicologic pathology integrates toxicology and the disciplines within it (such as biochemistry, pharmacodynamics and risk assessment) to pathology and its related disciplines (such as physiology, microbiology, immunology, and molecular biology).

Fundamentals of Toxicologic Pathology Second Edition updates the information presented in the first edition, including five entirely new chapters addressing basic concepts in toxicologic pathology, along with color photomicrographs that show examples of specific toxicant-induced diseases in animals. The current edition also includes comparative information that will prove a valuable resource to practitioners, including diagnostic pathologists and toxicologists.
25% brand new information, fully revised throughout New chapters: Veterinary Diagnostic Toxicologic Pathology; Clinical Pathology; Nomenclature: Terminology for Morphologic Alterations; Techniques in Toxicologic Pathology New color photomicrographs detailing specific toxicant-induced diseases in animals Mechanistic information integrated from both toxicology and pathology discussing basic mechanisms of toxic injury and morphologic expression at the subcellular, cellular, and tissue levels

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Matthew A. Wallig, Wanda M Haschek, Colin G. Rousseaux

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Natural Killer Cells explains the importance of killer cells and how they are produced. It mentions that the most likely explanation for killer cell production is that they serve as a complementary system for T cells as a primary defense against viruses. However, these cells defend against certain viruses only, such as herpes viruses and influenza viruses.

The book also explains the primary functions of killer cells, and it discusses how these cells help recognize damaged tissues, limit further damage to tissues, and regenerate damaged tissues. It discusses how these cells mature and develop, and it covers the different isolation, culture, and propagation methods of these cells. Furthermore, it focuses on the different killer cells that are present in various parts of the human body.

The book concludes by explaining that natural killer cells are utilized for clinical therapy of malignancies, and that they have led to positive outcomes in the field of biology and medicine.
Provides a broad, detailed coverage of the biology and interactions of NK cells for students, fellows, scientists, and practitioners Includes figures, histologic sections, and illustrations of the ontogeny of NK cells

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Medical Cell Biology, Third Edition, focuses on the scientific aspects of cell biology important to medical students, dental students, veterinary students, and prehealth undergraduates. With its National Board-type questions, this book is specifically designed to prepare students for this exam.

The book maintains a concise focus on eukaryotic cell biology as it relates to human and animal disease, all within a manageable 300-page format. This is accomplished by explaining general cell biology principles in the context of organ systems and disease.

This updated version contains 60% new material and all new clinical cases. New topics include apoptosis and cell death from a neural perspective; signal transduction as it relates to normal and abnormal heart function; and cell cycle and cell division related to cancer biology.
60% New Material! New Topics include: Apoptosis and cell dealth from a neural perspective Signal transduction as it relates to normal and abnormal heart function Cell cycle and cell division related to cancer biology All new clinical cases Serves as a prep guide to the National Medical Board Exam with sample board-style questions (using Exam Master(R) technology): www.exammaster.com Focuses on eukaryotic cell biology as it related to human disease, thus making the subject more accessible to pre-med and pre-health students

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Pharmacology meets the rapidly emerging needs of programs training pharmacologic scientists seeking careers in basic research and drug discovery rather than such applied fields as pharmacy and medicine.

While the market is crowded with many clinical and therapeutic pharmacology textbooks, the field of pharmacology is booming with the prospects of discovering new drugs, and virtually no extant textbook meets this need at the student level. The market is so bereft of such approaches that many pharmaceutical companies will adopt Hacker et al. to help train new drug researchers.

The boom in pharmacology is driven by the recent decryption of the human genome and enormous progress in controlling genes and synthesizing proteins, making new and even custom drug design possible. This book makes use of these discoveries in presenting its topics, moving logically from drug receptors to the target molecules drug researchers seek, covering such modern topics along the way as side effects, drug resistance, pharmacogenomics, and even nutriceuticals, one in a string of culminating chapters on the drug discovery process.

The book is aimed at advanced undergraduates and beginning graduate students in medical, pharmacy, and graduate schools looking for a solid introduction to the basic science of pharmacology and envisioning careers in drug research.
Uses individual drugs to explain molecular actions Full color art program explains molecular and chemical concepts graphically Logical structure reflecting the current state of pharmacology and translational research Covers such intricacies as drug resistance and cell death Consistent format across chapters and pedagogical strategies make this textbook a superior learning tool

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Miles Hacker, William S. Messer, Kenneth A. Bachmann

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Arthropods are the most numerous and diverse group of animals and studying them requires the use of specialized equipment and specific procedures. This text describes effective methods and equipment for collecting, identifying, rearing, examining, and preserving insects and mites, and explains how to store and care for specimens in collections. It also provides instructions for the construction of many kinds of collecting equipment, traps, rearing cages, and storage units, as well as updated and illustrated keys for identification of the classes of arthropods and the orders of insects. Such information not only aids hobbyists and professionals in preparing insect collections, but it has become essential in documenting and standardizing collections of entomological evidence in forensic as well as pest management sciences.

* Over 400 professionally drawn illustrations * Identification keys to find arthropod orders * Comprehensive reading list * Detailed glossary of terms

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The Mouse Nervous System provides a comprehensive account of the central nervous system of the mouse. The book is aimed at molecular biologists who need a book that introduces them to the anatomy of the mouse brain and spinal cord, but also takes them into the relevant details of development and organization of the area they have chosen to study. The Mouse Nervous System offers a wealth of new information for experienced anatomists who work on mice. The book serves as a valuable resource for researchers and graduate students in neuroscience.
Systematic consideration of the anatomy and connections of all regions of the brain and spinal cord by the authors of the most cited rodent brain atlases A major section (12 chapters) on functional systems related to motor control, sensation, and behavioral and emotional states A detailed analysis of gene expression during development of the forebrain by Luis Puelles, the leading researcher in this area Full coverage of the role of gene expression during development and the new field of genetic neuroanatomy using site-specific recombinases Examples of the use of mouse models in the study of neurological illness

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Charles Watson, George Paxinos, Luis Puelles