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Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research.   The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology.   The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-virus interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control.   Cell biologists and researchers will find this book invaluable.

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From Gene to Protein: Translation into Biotechnology

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From Gene to Protein: Translation into Biotechnology is the 15th volume in the continuing series under the title ""Miami Winter Symposia"". The theme of the symposium is the translation of the basic research findings into the practical application of biotechnology. This book summarizes methodology and its applications that lie behind the practical innovations.   The book starts with reviews of techniques of eukaryotic cell culture, hybridoma technology and uses, and the in vitro synthesis of DNA and its use in the generation of protein analogs. Considerable space is devoted to development of monoclonal antibodies that promises to be the dominating tool of medical technology, both for diagnosis and therapy. Cloning into eukaryotic cells and methods of increasing the levels of gene expression are included. These topics reflect areas of intensive research that have important commercial and clinical value. Core chapters describe biological activities of cloned gene products, including reports on trials with human subjects of interferon, human insulin, and growth hormone. A panel session on horizons in biotechnology is also provided, looking forward to the directions of future research and its applications.   Biotechnologists, cell biologists, scientists, researchers, teachers, and students will greatly benefit from this book.

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Ethylene in Plant Biology focuses on the role of ethylene in plant physiology and the interrelationship between ethylene, fruit ripening, and respiration. It summarizes the physiology, biochemistry, production, regulation, plant effects, metabolism, and mechanism of action of ethylene.   This book presents an introduction to basic chemistry of ethylene and available techniques for its sampling and analysis. Then, it discusses the rate, environmental conditions, and reactions involved in ethylene production. Chapter 4 examines the effects of herbicides and hormones, such as auxin, gibberellins, cytokinins, and abscisic acid, on ethylene production. Meanwhile, the next chapter studies the so-called stress ethylene phenomenon in plants. In particular, this book examines the role of insects, temperature, water, gamma-irradiation, and mechanical and chemical stimuli in stress ethylene. The biochemical aspects of ethylene are covered in the subsequent chapters. These include its role in growth and development of plant, phytogerontological activity, role in ethylene synthesis, respiration, pigmentation, and hormone regulation. Chapter 9 presents the activity of ethylene relative to other hydrocarbon analogs and dose-response relationships for a number of ethylene-mediated processes. The concluding chapters tackle the attachment of ethylene to its site of action, including epinasty, root initiation, intumescence formation, and floral initiation. A discussion on the issue of ethylene air pollution is included.   This book will be useful to both undergraduate students and professional workers, especially those who have background in plant anatomy, plant physiology, or biochemistry.

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Herbivorous Insects: Host-Seeking Behavior and Mechanisms addresses mechanisms of searching behavior leading ultimately to host location of herbivorous insects. It is divided into four sections, wherein the first two sections deal with neurophysiology and the diversity of behavioral induction cues. The third section covers the searching mechanisms as affected by insects' breadth of diet. The last part examines the evolutionary analysis of the behavioral and physiological adaptations in insect/host plant relations.

This book starts with an introduction to the chemical sensory system as it relates to host selection in general. This is followed by considerable discussions on host-seeking behavior and allied patterns in behavior. This text also includes the study of oviposition behavior in butterflies belonging to Papilionidae. The third section presents host selection and colonization by three insects within the saprophage-predator continuum, namely, Hylurgopinus rufipes, Scolytus multistriatus, and Pissodes strobi. The behaviors by which certain oligophagous insects locate and select food plants are also considered. The concluding part addresses the unifying theme and the diversity of responses of phytophagous insects to plants.

The book provides direction toward developing a unifying theme and improving the ability to unravel the complexities of insect/plant interactions. Behaviorists, ecologists, entomologists, evolutionary biologists, and physiologists will find this book invaluable.

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The Cannabinoids: Chemical, Pharmacologic, and Therapeutic Aspects

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The Cannabinoids: Chemical, Pharmacologic, and Therapeutic Aspects provides a comprehensive discussion of the various aspects of cannabis and its constituents.   The book is organized into six sections. Section I covers the clinical aspects of cannabis including the health aspects, impact on memory function, and the characteristics and treatment of marijuana abusers. Section II on chemical aspects includes studies on the chemistry and structure-activity relationships of cannabinoids; smoking characteristics of marijuana cigarettes; and developments in cannabinoid analyses of body fluids. Section III on metabolic and pharmacokinetic aspects includes studies on the metabolism, disposition, and pharmacokinetics of delta-9-tetrahydrocannabinol (THC) in men and women; single dose kinetics of cannabidiol in man; and distribution and disposition of THC in different tissues of the rat.   Section IV on reproductive aspects include studies on the effects of chronic administration of THC on the early embryonic development of mice; effects of cannabinoids on spermatogenesis in mice; and possible mechanism for the cellular effects of marijuana on male reproductive function. Section V on neuropharmacologic aspects includes studies on the discriminative stimulus properties of THC and the effects of cannabinoids on neurotransmitter receptors in the brain. Section VI on therapeutic aspects includes studies such as the possible anxiolytic effects of cannabidiol; tetrahydrocannabinol effects on extrapyramidal motor behaviors in Parkinson's disease; and the use of cannabinoids in glaucoma.

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Applied Biochemistry and Bioengineering, Volume 2: Enzyme Technology discusses the industrial applications of immobilized enzymes.  Organized into 10 chapters, this volume first describes the techniques for the isolation and purification of intracellular and extracellular enzymes for use on an industrial scale. It then deals with immobilized enzyme processes, with an emphasis on immobilized glucose isomerase and the amylolytic enzymes related to the production of high-fructose syrups from starch. Significant topics on immobilized enzyme technology for future uses in energy transduction and in pharmaceutical modifications of steroid compounds are also explored.  Microbiologists, geneticists, and chemical engineers will find this book of great value.

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Cyclic, Linear, and High Polymeric Systems

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Phosphorus-Nitrogen Compounds: Cyclic, Linear, and High Polymeric Systems concerns itself with the chemistry of compounds containing alternating phosphorus - nitrogen atoms in the skeleton. The monograph aims to be an introduction to phosphorus-nitrogen chemistry, a review of advances in the field, and reference work.  The text is divided into three parts. Part I covers the introduction, historical background, and nomenclature of phosphorus-nitrogen compounds and the theories in bonding and structure of phosphazenes and phosphazanes. Part II deals with reactions such as the synthesis of the phosphorus-nitrogen skeleton, hydrolysis of phosphazenes and phosphazanes, and the aminolysis of halophosphazenes. Part III discusses polymer chemistry and includes topics such as polymerization, depolymerization, and phosphazene polymers.  The book is recommended for students and practitioners in the field of chemistry, especially those concerned with phosphorus nitrogen compounds and polymeric systems.

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Newer Methods of Nutritional Biochemistry V5

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Newer Methods of Nutritional Biochemistry: With Applications and Interpretations, Volume V, presents discussions and reviews of procedures that may have a significant impact on the future progress of the science of nutrition.  Comprised of seven chapters, this book discusses the nutritional and metabolic aspects of circadian rhythms; the relationship of amino acid requirements in terms of amino acid composition and availability from various food sources; and the characteristics of protein-calorie malnutrition. It also describes methods, biochemical mechanisms, and dietary factors that influence the metabolic conversion of dietary carbohydrates into lipid moieties. The book examines the influence of nutritional factors on ribosomal dynamics and discusses the isolation, physical, and biochemical characteristics of proteinase inhibitors found in soy and lima beans and other edible vegetable seeds. A novel method for determining the biological value of protein foodstuffs is also included.  This book will be a valuable resource for graduate students and investigators in nutrition and other life sciences.

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Newer Methods of Nutritional Biochemistry V4

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Newer Methods of Nutritional Biochemistry: With Applications and Interpretations, Volume IV, presents discussions and reviews of principles and procedures of nutritional biochemistry which have been developed for assays of nutritive quality of foods.  Comprised of six chapters, this book describes determinations of dietary needs of fats, vitamins, and amino acids which fail to apply the long-known ""Law of Diminishing Returns"" to the experimental data. It examines the correlation of urinary metabolites with dietary conditions from the point of view of the dynamic state of metabolism. The book also discusses analytical methods for determining plasma amino acids and their application to nutritional problems of young children; laboratory methods for evaluating changes in protein quality; optimal nutrition for the aged and basic mechanisms of biological aging; and advances in instrumentation and methodology and their application in resolving biological and nutritional problems.

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Advances in Magnetic Resonance: The Waugh Symposium, Volume 13 is a collection of manuscripts presented at the 1989 conference on High Resolution NMR in Solids at Massachusetts Institute of Technology. This conference recognizes the accomplishments of John S. Waugh and his colleagues in the entire field of high resolution nuclear magnetic resonance (NMR) in solids.  Organized into 11 chapters, this book first discusses the principles, limitations, and applications of single crystal, deuterium, proton, and rotational-echo double resonance NMR techniques. It then deals with other NMR methods, such as two-dimensional, electrophoretic, and site-selective excitation NMR, with particular emphasis on their theoretical background and application in solid systems. Other chapters describe several hybrid two-dimensional experiments leading to a significant increase in spectral quality. Discussions on the advantages of utilizing two-dimensional solid-state NMR in studying the complex and highly orientable biological samples such as DNA are provided in the concluding chapters of the book. This volume also provides the 113Cd NMR results in the prediction of the 113Cd shielding tensor orientation in the real ligand geometry at a metal site.  Analytical and quantum chemists, physicists, biochemists, and materials science researchers will find this book invaluable.

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Newer Methods of Nutritional Biochemistry V3

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Newer Methods of Nutritional Biochemistry: With Applications and Interpretations, Volume III, provides a compilation of biochemical procedures which have extensive applications in nutrition research. The focus is on simple procedures to evaluate the utilization of dietary proteins given the pressing problems in emergency feeding of populations in developing countries.  Comprised of nine chapters, this book discusses the nutritional and metabolic implications of changes in urinary amino acid levels. It examines the concept, role, and implications of protein reserves in the young and adult subjects. It also describes procedures which have contributed to the development of in vitro methods for the evaluation of protein quality. The book also discusses plant protein resources; lipoprotein transport; chemical assay of adrenocorticosteroids; studies of zinc metabolism; and folates in human nutrition.

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Organization and Assembly of Plant and Animal Extracellular Matrix

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Organization and Assembly of Plant and Animal Extracellular Matrix presents a state-of-the-art view of some of the experimental systems in plant and animal matrix biology. It discusses certain principles underlying establishment of complex three-dimensional architecture cross broad evolutionary boundaries.  The opening chapter reviews studies on the cellular mechanisms responsible for storage, release, assembly, and function of extracellular matrices during early sea urchin development. The subsequent chapters describe the structure, assembly, disassembly, and molecular biology of the Chlamydomonas reinhardtii cell wall. The chapters also summarize the status of work on basement membrane assembly. Important insights into approaches to identify critical molecular domains and the complexity of relating defined molecular associations to establishment of matrix architecture are provided. A family of discovered cell wall genes that encode protein products containing up to 70% glycine is presented in Chapter 4. This is followed by a discussion on the role of alginate self-assembly in cell wall formation in Fucus. The book goes on to address the issue of protein-carbohydrate recognition with a detailed discussion of plant and animal lectins. Chapter 7 tackles a family of genes encoding higher plant hydroxyproline-rich glycoproteins (HRGPs) and the relationship between the HRGP genes cloned and their products. The final two chapters are devoted to one of the most important classes of protein modifying enzymes for extracellular matrix formation and function, the prolyl hydroxylases.  This book will be of help to workers in plant and animal matrix in understanding information, approaches, and ideas that they may not normally encounter.

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Biological Events Probed by Ultrafast Laser Spectroscopy

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Biological Events Probed by Ultrafast Laser Spectroscopy summarizes the progress in the experimental and theoretical understanding of primary phenomena occurring in biology on a picoseconds and nanosecond time scale. This book includes basic principles, survey of research results, and thinking of experts in the fields of photosynthesis, vision, hemoglobin, and DNA.   This reference is organized into five parts. The primary events in the various areas of biology are reviewed in the first four parts. The last part covers the picosecond and subpicosecond laser techniques. Primary processes in photosynthesis are then discussed. This is followed by chapters on fluorescence and absorption kinetic measurements in higher plants. Subsequent chapters cover kinetic measurements in bacteria photosynthesis, theories of primary energy transfer appropriate to photosynthesis, and multiexcitation processes. Part II examines primary visual processes, including kinetic experimental work on rhodopsin and bacteriorhodopsin, and theoretical concepts on primary events in vision.  The third part presents the introductory materials on the kinetic models of hemoglobin and myoglobin. It also discusses the interesting picosecond measurements on these proteins. Part IV focuses on the application of modern ultrafast temporal techniques to the study of DNA and its components, as well as DNA-dye complexes. The concluding part introduces the principles of design and operation of the solid state laser and picoseconds spectroscopy methods. A discussion on pulse generation methods and measurement techniques applicable for measuring primary events in biological systems is provided.  This book is useful both for the newbies and experts in the field of ultrafast phenomena. It aims to attract biologists, chemists, physicists, and engineers who are interested in biological processes. It will help readers find all the necessary and relevant material in one presentation.

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Control and Dynamic Systems: Advances in Theory in Applications, Volume 29: Advances in Algorithms and Computational Techniques in Dynamic Systems Control, Part 2 of 3 discusses developments in algorithms and computational techniques for control and dynamic systems.   This volume discusses some computational problems which arose in the applications of Kalman filters. It also examines system fault detection techniques; computational techniques in angle-only tracking filtering; development of real-time knowledge of system parameters; and algorithms for decentralized systems with application to stream water quality.  This book is an important reference for practitioners in the field who want a comprehensive source of techniques with significant applied implications.

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Introduction to the Mathematics of Quasicrystals

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Introduction to the Mathematics of Quasicrystals provides a pedagogical introduction to mathematical concepts and results necessary for a quantitative description or analysis of quasicrystals. This book is organized into five chapters that cover the three mathematical areas most relevant to quasicrystals, namely, the theory of almost periodic functions, the theory of aperiodic tilings, and group theory.  Chapter 1 describes the aspects of the theory of tiling in two- and three-dimensional space that are important for understanding some of the ways in which classical mathematical crystallography is being generalized; this process is to include possible models for aperiodic crystals. Chapter 2 examines the non-local nature of assembly mistakes that might have significance to the quasicrystals growth. This chapter also describes how closely a physical quasicrystal might be able to approximate a three-dimensional version of tilings. Chapter 3 discusses the theoretical background and concepts of group theory of icosahedral quasicrystals. Chapter 4 presents the local properties of the three-dimensional Penrose tilings and their global construction is described through the projection method. This chapter emphasizes the relationship between quasiperiodic sets of points and quasiperiodic tiling. Chapter 5 explores the analysis of defects in quasicrystals and their kinetics, as well as some properties of the perfect system.  This book is of great value to physicists, crystallographers, metallurgists, and beginners in the field of quasicrystals.

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Comparative Transfusion Medicine describes the role of animals as donors in early human transfusions. Organized into 11 chapters, the book focuses on specific animal models of human hematologic diseases.  After briefly dealing with the history of transfusion in medicine, the book discusses erythrocytes, white cells, platelets, and coagulation in various animal species, and then describes specific animal models of human hematologic diseases. It then considers the progress in bone marrow transplantation by pioneering histocompatibility studies of dogs. The discussion then shifts to the preparation components and clinical veterinary transfusions. The book also presents three problems in neonatal transfusion, including the failure of passive transfer, isoerythrolysis, and immunotherapy. The concluding chapters explore the developments in human autologous transfusion, blood substitutes, and hematopoietic growth factors.  The book is of great value to veterinarians involved in research or in clinical transfusions, and to physicians and other scientists using animals in research.

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Advances in Magnetic Resonance focuses on the interdisciplinary field of magnetic resonance.  Comprised of four chapters, this book discusses collective atomic motions in crystals as studied by nuclear magnetic resonance (NMR) spectroscopy and elaborates Mori's formalism as applied to the spin relaxation theory. It also discusses chemically induced dynamic nuclear polarization, magnetic shielding, and magnetic susceptibility.  Students and physicists looking for a comprehensive source on magnetic resonance will find this book invaluable.

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Control and Dynamic Systems V54: System Performance Improvement and Optimization Techniques and Their Applications in Aerospace Systems

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Control and Dynamic Systems: Advances in Theory and Applications, Volume 54: System Performance Improvement and Optimization Techniques and their Applications in Aerospace Systems covers the issue of aerospace system performance and optimization techniques in aerospace systems.  This book is composed of 12 chapters and begins with an examination of the techniques for aircraft conceptual design for mission performance. The succeeding chapters describe the balances and optimized design for aircraft and spacecraft structures through finite element procedures and the application of the knowledge-based system techniques for pilot aiding. These topics are followed by discussions of the optimal sensor placement for on-orbit modal identification experiments; the optimization techniques for helicopter airframe vibrations design; the size reduction techniques for efficient aeroservoelastic model determination; sensitivity analysis of eigendata of aeroelastic systems; and a simplified solution for transient structural dynamic problems with local nonlinearities. Other chapters explore a reduction algorithm for systems with integrators and the techniques for overcoming the difficulty of nonuniqueness of mode shape in modal analysis when random input data are not or cannot be measured. The last chapters consider the combined concepts of Krylov vectors and parameter matching and their application to develop model-reduction algorithms for structural dynamics. These chapters also provide the techniques for the development of new tracking algorithms that would incorporate explicit models of the maneuvering/nonmaneuvering phases of target encounter.  This book will prove useful to aerospace, control, systems, and design engineers.

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Advances in High Temperature Chemistry V4

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Advances in High Temperature Chemistry, Volume 4 reviews and evaluates some techniques in high temperature chemistry.   This book first discusses infrared and ultraviolet spectroscopy of free radicals and molecular ions. It then turns to atomic carbon reactions and gas-solid surface reactions. It also presents several techniques for studying liquids and solids at extreme temperatures.  Researchers and high temperature chemists will find this book invaluable.

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The Pretransition Theory of Vapor Condensation

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Homogeneous Nucleation Theory: The Pretransition Theory of Vapor Condensation discusses the influence of classical thermodynamics, statistical mechanics, and multistate kinetics on the homogeneous nucleation theory.  This book is organized into 10 chapters and begins with a simple model calculation that yields an important insight into the major physical features governing supersaturated vapor condensation. The following chapters explore the development of the theory of equilibrium thermodynamics pertinent to the study of a nucleation phenomena and a postulatory formulation of statistical mechanics and its relation to the calculation of the thermodynamic potentials. The discussion then shifts to a statistical thermodynamics description of an imperfect gas assuming the droplet model of Band-Bijl-Frenkel and to the development of the multistate kinetics of cluster formation. The book also explores the development of the classical Einstein theory for crystalline solids and generalizes this theory for its applications to planar surfaces of microcrystalline clusters. It also presents a comparison of the exact free energies for the microcrystallites with the predictions of the droplet model using the capillarity approximation. Three distinct approaches for calculating the thermodynamic properties of physical clusters are covered in the concluding chapters.