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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.

Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.

This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field.
Among the first books to cover principles and methodology of colloidal gold in microscopy Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book Discusses problems and limitations of techniques Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins Preserving and localizing antigenicity; sectioning; high-resolution morphological localization Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation Applications to biomedical studies Colloidal gold in conjunction with video-enhanced microscopy

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Written in a clear and simple style, this textbook on fluid mechanics gives equal emphasis to both geophysical and engineering fluid mechanics. For physicists, it contains chapters on geophysical fluid mechanics and gravity waves; for engineers, it has chapters on aerodynamics and compressible flow. Of common interest are chapters on governing equations, laminar flows, boundary layers, instability, and turbulence. This book also presents topics of recent interest, such as deterministic chaos, and double-diffusive instability.

n Gives equal treatment to topics in both engineering and geophysical fluid dynamicsn Suitable as an intermediate or graduate course textbook for students in their senior year or aboven Treats topics of recent interest such as deterministic chaos, double diffusive instability and solitonn Extensively illustratedn Contains fully worked examples in each chapter as well as end-of-chapter problemsn An instructor's manual is available

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This book appears at a time when molecular cytogenetics is positioned to make a significant impact upon evolutionary studies, enabling problems of chromosomal structure and change to be critically assessed. It is an up-to-date and comprehensive survey of the cytogenetics of a major class of animals, including all three amphibian orders, with chapters authored by international leaders in the field.Amphibian Cytogenetics and Evolution will be of interest to classical and molecular cytogeneticists, systematicists, evolutionary biologists, herpetologists, and anyone using amphibians in genetic research. Offers the only current and comprehensive survey of amphibian cytogenetics Gives authoritative and in-depth coverage of topics of present interest Reviews general cytogenetic topics Presents new insights into evolutionary changes in chromosome structure and amphibian phylogeny and relationships including: Phylogenetic analysis of chromosome data, Current techniques of cytogenetic analysis, Examination of all three amphibian orders

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An Introduction to Programming with Specifications

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A feature of modern advanced computing is the functional approach to programming. In this book, the authors present an introduction to the mathematics which underline functional programming, emphasizing the understanding of definition and specification--a prerequisite of good programming and problem solving with a computer. The book is self-contained, requiring a low level of mathematical sophistication and may be used as an introduction to the mathematics of programming.

Provides an introduction to the functional approach to programming**Emphasizes the problem to be solved, not the programming language**Takes the view that all computer programs are a definition of a function**Includes exercises for each chapter**Can be used as a pre-programming language introduction to the mathematics of computing.

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Dietary fats and carbohydrates represent some eighty to ninety percent of food energy uptake in man; fatty acids play a critical role in human development, health and disease. In affluent populations high fat consumption contributes to heart disease, obesity and type II diabetes mellitus, while in non-affluent groups, the generally poor nutritional state found in young children can be partially attributed to a low fat intake.<br>This book reviews our current understanding of essential fatty acids and their role in human nutrition. The topics addressed include the analysis of dietary fatty acids, dietary fats and fish oils in health and in the prevention of heart disease, linoleic acid in the treatment of diabetes, and the role of essential fatty acids in early human development.

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Introduction to Forest and Shade Tree Insects

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This comprehenisive text approaches the subject from an ecological/evolutionary biological perspective. The assumption is that one cannot study forest insects without understanding the dynamics of the relationship between an insect and its host plant. This relationship includes knowing what factors control forest insect populations such as food, food quality, tree vigor, host selection, and symbiotic relationships. The authors also discuss tree-injuring insects from the perspective of their influence on tree physiology and growth as well as economic and commercial effects. The book represents a "modern" approach to the topic of forest and shade tree insects; is well-illustrated; and includes a comprehensive primary reference list.

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Here is a comprehensive, practical guide to the entire process of analog instrumentation and control, from sensor input to data conversion circuitry and final output. This readable handbook avoids complex mathematical treatments, instead taking an applications-oriented approach and presenting many sample circuits and concrete examples. It is an essential reference for engineers and high-level technicians in a variety of scientific and engineering fields--anywhere data is collected electronically and where such data is used to control physical processes. Covers design of instrumentation, control systems, and data acquisition circuits Explains standard devices and techniques in a convenient, well-organized format Takes an applications-oriented approach, rather than a theoretical one

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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books Problems and limitations of techniques are discussed Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields

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Major developments have taken shape in the ten years since the publication of Plant Virology, Second Edition. This Third Edition of the leading comprehensive text and reference for the field contains more than sixty percent new material, including applications and results of gene manipulation techniques. As with the first and second editions, this volume covers all aspects of plant virology, from molecular to ecological. Plant Virology, Third Edition, is intended for graduate students, researchers, and teachers in plant virology, plant pathology, general virology, and microbiology, and scientists in related areas of molecular biology, biochemistry, plant physiology, and entomology.

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Their Role in Conservation and Development

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This book includes the proceedings of the second symposium on one important role of botanic gardens in conserving world plant resources. Sessions covered topics such as implementing the botanic gardens conservation strategy, regional networks and national programs in the tropics, and germplasm banks in botanic gardens.

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For students as well as researchers this book describes the exciting new advances in the molecular biology of transport proteins and integrates this information with transport kinetics, function, and regulation. Experimental data are linked with theory. Provides an introduction to the properties of transport proteins: channels, carriers, and pumps Presents up-to-date information on the structure of transport proteins and on their function and regulation Includes introductions to transport kinetics and to the cloning of genes that code transport proteins Furnishes a link between the experimental basis of the subject and theoretical model building

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Pathology in Marine Science contains the majority of papers presented at the Third International Colloquium on Pathology in Marine Aquaculture held in Gloucester Point, Virginia, USA in October 1988.  The book serves as a record of the progress of concerted research in marine pathobiology and also as a useful reference tool. The compendium consists of contributions that are reflective of the subdisciplines of the biological sciences that are of immediate concern to investigators interested in pathology in marine aquaculture. Topics discussed include viruses, bacterioses, mycoses, protozoan diseases, metazoan parasitic diseases, toxicological syndromes, teratological and neoplastic diseases, epidemiology/epizootiology, nutritional pathology, and immunology.  Marine scientists, aquaculturists, and researchers on marine life science will find the text useful.

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This text is the successor volume to Biophysical Plant Physiology and Ecology (W.H. Freeman, 1983). The content has been extensively updated based on the growing quantity and quality of plant research, including cell growth and water relations, membrane channels, mechanisms of active transport, and the bioenergetics of chloroplasts and mitochondria. One-third of the figures are new or modified, over 190 new references are incorporated, the appendixes on constants and conversion factors have doubled the number of entries, and the solutions to problems are given for the first time. Many other changes have emanated from the best laboratory for any book, the classroom.

Covers water relations and ion transport for plant cells; diffusion, chemical potential gradients, solute movement in and out of plant cells Covers interconnection of various energy forms; light, chlorophyll and accessory photosynthesis pigments, ATP and NADPH Covers forms in which energy and matter enter and leave a plant; energy budget analysis, water vapor and carbon dioxide, water movement from soil to plant to atmosphere

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This up-to-date compilation addresses the scientific problems of determining a mode of action of lithium and provides a cross-disciplinary perspective. In addition to psychiatrists interested in the therapeutic uses of lithium, it will also be of interest to biochemists using lithium as an inhibitor of phosphoinositide signaling, immunologists studying lymphocytes, and dermatologists studying viral replication in the skin.

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Adsorption of Gases on Heterogeneous Surfaces

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All real solid surfaces are heterogeneous to a greater or lesser extent and this book provides a broad yet detailed survey of the present state of gas adsorption. Coverage is comprehensive and extends from basic principles to computer simulation of adsorption. Underlying concepts are clarified and the strengths and weaknesses of the various methods described are discussed. Adsorption isotherm equations for various types of heterogeneous solid surfaces Methods of determining the nature of surface heterogeneity and porosity from experimental data Studies of phase behavior of gases absorbed on heterogeneous solid surfaces Computer simulation of adsorption on heterogeneous solid surfaces

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Using your Computer to Develop and Diagnose Feedback Controllers

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This is a practical approach to control techniques. The author covers background material on analog controllers, digital controllers, and filters. Commonly used controllers are presented. Extended use of PSpice (a popular circuit simulation program) is used in problem solving. The book is also documented with 50 computer programs that circuit designers can use. Explains integration of control systems with a personal computer Compares numerous control algorithms in digital and analog form Details the use of SPICE in problem solving Presents modeling concepts for linear and nonlinear systems Examines commonly used controllers

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A Guide to PLDs, State Machines, and Microcontrollers

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This is a practical guide to programmable logic devices. It covers all devices related to PLD: PALs, PGAs, state machines, and microcontrollers. Usefulness is evaluated; support needed in order to effectively use the devices is discussed. All examples are based on real-world circuits.

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The arctic region is predicted to experience the earliest and most pronounced global warming response to human-induced climatic change. This book synthesizes information on the physiological ecology of arctic plants, discusses how physiological processes influence ecosystem processes, and explores how climate warming will affect arctic plants, plant communities, and ecosystem processes. Reviews the physiological ecology of arctic plants Explores biotic controls over community and ecosystems processes Provides physiological bases for predicting how the Arctic will respond to global climate change

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F. Stuart Chapin III, Robert L. Jefferies, James F. Reynolds, Gaius R. Shaver, Josef Svoboda, Ellen W. Chu

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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.

This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.
Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry Methods for preparing colloidal gold particles of different sizes Protein A-gold, protein G-gold, and lectin-gold techniques The use of resins and thin cryosections Multiple labeling

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Handbook of Flexible Manufacturing Systems

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This handbook is a compilation of the current practical knowledge of flexible manufacturing systems (FMS). FMS allow manufacturing plants of all sizes to reduce their inventory while increasing their ability to meet consumer demands. By controlling automatic guided vehicles, robots, and machine tools with one central computer, products can now be produced in a variety of styles and models all at the same time. FMS are designed to adapt quickly and economically to changes in requirements and to unpredictable events. This guide explains how to effectively employ these useful new systems. Includes specifications for software to implement simulation modeling Surveys practical applications in the workplace Presents materials in a step-by-step workbook style