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Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry.

Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher.
Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors

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Ray Wu, Lawrence Grossman, Kivie Moldave

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Although many texts exist offering an introduction to artificial intelligence (AI), this book is unique in that it places an emphasis on knowledge representation (KR) concepts. It includes small-scale implementations in PROLOG to illustrate the major KR paradigms and their developments.****back cover copy:**Knowledge representation is at the heart of the artificial intelligence enterprise: anyone writing a program which seeks to work by encoding and manipulating knowledge needs to pay attention to the scheme whereby he will represent the knowledge, and to be aware of the consequences of the choices made.****The book's distinctive approach introduces the topic of AI through a study of knowledge representation issues. It assumes a basic knowledge of computing and a familiarity with the principles of elementary formal logic would be advantageous.****Knowledge Representation: An Approach to Artificial Intelligence develops from an introductory consideration of AI, knowledge representation and logic, through search technique to the three central knowledge paradigms: production rules, structured objects, and predicate calculus. The final section of the book illustrates the application of these knowledge representation paradigms through the Prolog Programming language and with an examination of diverse expert systems applications. The book concludes with a look at some advanced issues in knowledge representation.****This text provides an introduction to AI through a study of knowledge representation and each chapter contains exercises for students. Experienced computer scientists and students alike, seeking an introduction to AI and knowledge representations will find this an invaluable text.

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Game Theory and Applications outlines game theory and proves its validity by examining it alongside the neoclassical paradigm. This book contends that the neoclassical theory is the exceptional case, and that game theory may indeed be the rule. The papers and abstracts collected here explore its recent development and suggest new research directions. Explains many of the recent central developments in game theory Highlights new research directions in economic theory which surpass the neoclassical paradigm Includes game-theoretical analyses in economics, political science, and biology Written by leading game theorists, economists, political scientists, and biologists

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Tatsuro Ichiishi, Abraham Neyman, Yair Tauman, Karl Shell

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Heterostructure and quantum-mechanical devices promise significant improvement in the performance of electronic and optoelectronic integrated circuits (ICs). Though these devices are the subject of a vigorous research effort, the current literature is often either highly technical or narrowly focused. This book presents heterostructure and quantum devices to the nonspecialist, especially electrical engineers working with high-performance semiconductor devices. It focuses on a broad base of technical applications using semiconductor physics theory to develop the next generation of electrical engineering devices. The text covers existing technologies and future possibilities within a common framework of high-performance devices, which will have a more immediate impact on advanced semiconductor physics-particularly quantum effects-and will thus form the basis for longer-term technology development.

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Norman G. Einspruch, William R. Frensley

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The Sociolinguistics of the Deaf Community

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This is a unified collection of the best and most current empirical studies of socio-linguistic issues in the deaf community, including topics such as studies of sign language variation, language contact and change, and sign language policy.

Established linguistic concerns with deaf language are reexamined and redefined, and several new issues of general importance to all sociolinguists are raised and explored. This is a book which interests all sociolinguists as well as deaf professionals, teachers of the deaf, sign language interpreters, and anyone else dealing on a day-to-day basis with the everyday language choices that deaf persons must make.

This is a unified collection of the best and most current empirical studies of sociolinguistic issues in the deaf community, including topics such as:
Studies of Sign Language Variation Language contact and Change Sign Language Policy Language Attitudes Sign Language Discourse Analysis

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Advances in Software Science and Technology

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This serial is a translation of the original works within the Japan Society of Software Science and Technology. A key source of information for computer scientists in the U.S., the serial explores the major areas of research in software and technology in Japan. These volumes are intended to promote worldwide exchange of ideas among professionals.This volume includes original research contributions in such areas as Augmented Language Logic (ALL), distributed C language, Smalltalk 80, and TAMPOPO-an evolutionary learning machine based on the principles of Realtime Minimum Skyline Detection.

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Knowledge-Based Systems and Legal Applications

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This book compiles the experience of the largest project in knowledge-based systems and the law yet undertaken. It provides an in-depth introduction to representation of law in computer programs, as well as more advanced discussion and description of large knowledge-based systems building, legal representation, cooperative work, and interface design in the context of the project. Describes the world's largest KBS and law project Contains an authoritative survey of approaches to legal knowledge representation Outlines several prototype systems Discusses the integration of KBS and law issues with HCI and social implications

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This book describes techniques for designing and building adaptive user interfaces developed in the large AID project undertaken by the contributors. Describes one of the few large-scale adaptive interface projects in the world Outlines the principles of adaptivity in human-computer interaction

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Molecular Basis of Mitochondrial Pathology

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The field of mitochondrial diseases is currently one of the rapidly growing fields of research in cell and molecular biology. This volume encompasses the latest development in this field of research. The chapters cover topics in a wide range of disciplines including biophysics, biochemistry, cell and molecular biology, molecular genetics, and clinical medicine. Summarizes growing evidence of the role of mitochondria in a large number of pathological conditions Brings together different approaches toward understanding mitochondria diseases Molecular and cellular biology Clinical physiology and medicine Details the crucial role this organelle plays in genetic regulation of various biological functions

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Advances in Archaeological Method and Theory

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Advances in Archaeological Method and Theory

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Topics in the Analysis of Archaelogical Faunas

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Planning and Design of Information Systems

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Planning and Design of Information Systems provides a theoretical base and a practical method of executing the planning of computerized information systems, and the planning and design of individual applications. The book is organized into five parts, covering the non-technical and nonimplementational part of information systems planning, design, and development. Part I gives the theoretical base for the subsequent parts of the book. It discusses modeling, techniques, notations, boundaries, quality issues and aspects, and decomposition techniques and problems. Part II discusses the needs, problems, and possible solutions for user participation. It describes user organizations, in respect of culture; maturity in the use of automation and computers; and gives a synthesis of participation, cultures, maturity, and information systems development techniques. Part III describes how to develop an Information Systems Architecture, an Information Systems Plan, and a Data Architecture. Part IV presents a structured, programmed approach to planning an application in a short period while maintaining high quality, and discusses project management of application development. Part V covers information analysis, data specification and conceptual data base design, and process and dialog design.

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This book is intended to provide a somewhat more comprehensive and unified treatment of large sample theory than has been available previously and to relate the fundamental tools of asymptotic theory directly to many of the estimators of interest to econometricians. In addition, because economic data are generated in a variety of different contexts (time series, cross sections, time series--cross sections), we pay particular attention to the similarities and differences in the techniques appropriate to each of these contexts.

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Handbook of Mathematical Formulas and Integrals

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If there is a formula to solve a given problem in mathematics, you will find it in Alan Jeffrey's Handbook of Mathematical Formulas and Integrals. Thanks to its unique thumb-tab indexing feature, answers are easy to find based upon the type of problem they solve. The Handbook covers important formulas, functions, relations, and methods from algebra, trigonometric and exponential functions, combinatorics, probability, matrix theory, calculus and vector calculus, both ordinary and partial differential equations, Fourier series, orthogonal polynomials, and Laplace transforms. Based on Gradshteyn and Ryzhik's Table of Integrals, Series, and Products, Fifth Edition (edited by Jeffrey), but far more accessible and written with particular attention to the needs of students and practicing scientists and engineers, this book is an essential resource. Affordable and authoritative, it is the first place to look for help and a rewarding place to browse.

Special thumb-tab index throughout the book for ease of useAnswers are keyed to the type of problem they solveFormulas are provided for problems across the entire spectrum of MathematicsAll equations are sent from a computer-checked source codeCompanion to Gradshteyn: Table of Integrals, Series, and Products, Fifth EditionThe following features make the Handbook a Better Value than its Competition:Less expensiveMore comprehensiveEquations are computer-validated with Scientific WorkPlace(tm) and Mathematica(r)Superior quality from one of the most respected names in scientific and technical publishingOffers unique thumb-tab indexing throughout the book which makes finding answers quick and easy

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Data Acquisition Techniques Using Personal Computers contains all the information required by a technical professional (engineer, scientist, technician) to implement a PC-based acquisition system. Including both basic tutorial information as well as some advanced topics, this work is suitable as a reference book for engineers or as a supplemental text for engineering students. It gives the reader enough understanding of the topics to implement a data acquisition system based on commercial products. A reader can alternatively learn how to custom build hardware or write his or her own software. Featuring diverse information, this book will be useful to both the technical professional and the hobbyist. Contains tables of reference information on PC/XT/AT computers that are usually not found in a single source Includes hardware information, such as I/O addresses, memory maps, and hardware interrupts Discusses software reference material including BIOS and DOS interrupt calls Presents valuable hardware interface information including timing diagrams, design examples, and descriptions of standard interfaces, such as the RS-232 serial interface

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The Third World Congress on Biosensors Abstracts

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The Third World Congress on Biosensors Abstracts